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Multimodal Analysis of Human Layer 5 Pyramidal Neurons

This dataset includes intrinsic membrane properties of 113 human L5 pyramidal neurons acquired via patch-clamp recordings in brain slices from the temporal cortex region. We also collected the transcriptome (n=25) and/or dendritic morphologies (n=15) from a subset of these cells. Together this dataset reveals two broad classes of neurons with transcriptomic and morpho-electric properties resembling extratelencephalic- and intra-telencephalic projecting neurons.  

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File Description

Cell metadata and electrophysiology features

Excel spreadsheet with cell metadata, computed electrophysiology features, and dictionary describing the fields metadata fields and features.

Gene Expression Matrix

CSV with expression values for 25 cells (counts per million).

Link to data archive for raw transcriptomics files

Coming soon


This work was funded by the Allen Institute for Brain Science and also supported in part by the U.S. National Institutes of Health (NIH) grant U01 MH114812-02 to E.S.L., NIH BRAIN Initiative award RF1MH114126 from the National Institute of Mental Health to E.S.L., J.T.T., and B.P.L., NIH BRAIN Initiative RF1MH121274 and NIH R01DA036909 awards to B.T. NIA grant AG005136 to the UW ADRC Neuropathology Core, a grant from the Nancy and Buster Alvord Endowment to C.D.K., and NIH grants P51OD010425 from the Office of Research Infrastructure Programs (ORIP) and UL1TR000423 from the National Center for Advancing Translational Sciences (NCATS).