image representing the current explore topic

Ophys/Ephys Calibration Data

Spiking activity and simultaneously recorded fluorescence signals in transgenic mice expressing GCaMP6

 

We present a dataset consisting of simultaneously measured fluorescence and spiking activity of pyramidal neurons in layer 2/3 of primary visual cortex in transgenic mouse lines expressing genetically-encoded calcium indicators (GECIs) GCaMP6s or GCaMP6f.

 

The dataset consists of 91 neurons from 4 transgenic mouse lines for which calcium imaging was performed at high optical zoom focused on individual cells. A subset (37) of these neurons were additionally imaged at a lower zoom factor, i.e. one at which the responses of many neurons can be characterized in parallel. The dataset is described in Huang, Knoblich et al., Relationship between spiking activity and simultaneously recorded fluorescence signals in transgenic mice expressing GCaMP6.

Publication | Data

 

All data and code are covered by the Allen Institute Terms of Use. By making this data available, we hope that it will serve the community as (1) a further resource to better understand quantitatively the link between calcium-evoked fluorescence imaging signals and spiking activity; and (2) a ground truth dataset for inferring spiking activity from calcium imaging in transgenic mouse lines.