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Toolkit

Transgenic Mouse Lines

The Allen Institute for Brain Science has generated a large collection of transgenic mouse lines for use in research. Driver lines enable access to genetically-identified cell classes and can be used in combination with reporter lines to visualize or modify diverse populations of cells.  This suite of molecular tools is used in our research and lines are made available to the community via The Jackson Laboratory.

 

Characterization data legend: Phenotypic characterization of selected lines can be accessed below, by clicking on the colored circle corresponding to a mouse line and a data modality code:  Histology:  Gene expression data by in situ hybridization (ISH)         - Neuronal connectivity:  Projectional maps         - In vivo activity:  Sensory-evoked calcium responsiveness       

Driver lines

 

 

 

 

Reporter lines

 

Abbreviations:  RCL, Rosa26 – CAG promoter – LoxP-STOP-LoxP. RCFL, Rosa26 – CAG promoter – FRT-STOP-FRT – LoxP-STOP-LoxP. RCRL, Rosa26 – CAG promoter – Rox-STOP-Rox – LoxP-STOP-LoxP. RCF, Rosa26 – CAG promoter – FRT-STOP-FRT. RCR, Rosa26 – CAG promoter – Rox-STOP-Rox. LSL, LoxP-STOP-LoxP. TITL, TIGRE – Insulators – TRE promoter – LoxP-STOP-LoxP. TIT, TIGRE – Insulators – TRE promoter. TIT2L, TIGRE – Insulators – TRE2 promoter – LoxP-STOP-LoxP. ICL, Insulators – CAG promoter – Lox2272-STOP2-Lox2272. ICR, Insulators – CAG promoter – Rox-STOP2-Rox. FnGF, FRT – nls-mNeonGreen – FRT. GC6f, GCaMP6f. GC6s, GCaMP6s. nT, nls-tdTomato. Syp, Synaptophysin. tdT, tdTomato. TPT, tdTomato-P2A-tTA2.