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Transcriptional Landscape of the Brain

The Allen Institute has produced numerous datasets to map the location and intensity of gene expression across the normal and diseased central nervous system, throughout development, and in multiple species. Together these data, tools and publications provide insight into the transcriptional landscape of the mammalian brain.  Browse these resources below.

The genetic geography of the brain

The patterning of gene usage across the anatomical structures of the adult human brain is highly stereotyped and reproducible. This dynamic heatmap allows users to explore shared patterning across individuals. The 'top genes' that are differentially expressed between any two brain regions can be accessed via links to view the data and analyze functional enrichment using the ToppGene suite from the Cincinnati Children's Hospital Medical Center.

Explore the relationship between gene expression and anatomy  |  Access the data  |  ToppGene: Explore gene enrichments

Single cell transcriptomics in human and mouse brain

Single cell (or single nucleus) RNA sequencing (RNA-Seq) is a scalable approach to provide genome-wide expression profiles for thousands of cells. This dataset includes single-cell and -nucleus transcriptomic profiles, assayed from human and mouse brain regions. Anatomical specificity is achieved by microdissecting tissue from defined brain areas, such as cortical layers or cell groups in lateral geniculate nucleus (LGd).

Download the data  |  Browse human data  |  Browse mouse data  |  Documentation

Data visualization tool for transcriptional profiles of human mediotemporal gyrus (MTG)

The Allen Cell Types Database contains RNA-Seq data from over 15,000 cell nuclei.  These data are navigable via Cytosplore, a downloadable application to interactively explore the cellular hierarchy of human MTG at the transcriptional level. This application is made in collaboration with the Leiden Computational Biology Center, the Division of Image Processing at the Leiden University Medical Center, and the Computer Graphics and Visualization Group at the TU Delft.

MTG Viewer in Cytosplore

The NIH BRAIN Initiative: Access diverse data from human, mouse, and marmoset brain

The National Institute of Health (NIH) Brain Research though Advancing Innovative Neurotechnologies (BRAIN) Initiative - Cell Census Network (BICCN) aims to provide researchers and the public with a comprehensive reference of the diverse cell types in human, mouse, and marmoset brain.  Transcriptomic data from these projects, which includes data from the Allen Institute, are available via the Brain Cell Data Center (BCDC) Portal.

BCDC portal  |  Data access

Comprehensive gene expression atlas of adult mouse brain

Introduced more than 10 years ago, this genome-wide, high-resolution atlas of gene expression throughout the adult mouse brain remains our most used atlas.  The atlas includes in situ hybridization data of ~20,000  genes spanning the entire mouse brain at cellular resolution. Quantitative image data is visualized spatially in 3D.

View data  |  Documentation  |  API  |  Help  

Comprehensive gene expression atlas of adult human brain

The Allen Human Brain Atlas is a unique multimodal atlas of the human brain, integrating anatomic and genomic information. It includes microarray data providing an "all genes, all structures" survey in multiple adult control brains and in situ hybridization image data comprising multiple datasets from disease and control cases and totaling over 1000 genes.

View data  |  Documentation  |  Help  

Gene expression atlas of mouse brain development

The Allen Developing Mouse Brain Atlas project surveyed gene expression in the brain beginning with mid-gestation through to juvenile and young adult. This 4D in situ hybridization atlas provides a framework to explore temporal and spatial regulation of gene expression in mouse brain for approximately 2,000 genes across seven stages of development.

View data  |  Documentation  |  API  |  Help  

Gene expression atlas of the developing human brain

The NIH BrainSpan project is a foundational resource for studying transcriptional mechanisms involved in human brain development. It includes (1) RNA sequencing and exon microarray data profiling of 16 cortical and subcortical structures across human brain development; (2) high-resolution neuroanatomical transcriptional profiles of ~300 distinct structures spanning the entire brain in midgestation; and (3) in situ hybridization image data covering selected genes and brain regions.

Developmental transcriptome  |  Microarray data  |  In situ hybridization  |  Documentation

Transcriptional profiling of the developing non-human primate brain

The NIH Blueprint Non-Human Primate (NHP) Atlas consists of a suite of gene expression data, neuroanatomical data and informatics tools for exploring the cellular and molecular architecture of the developing rhesus macaque brain. The high-resolution transcriptional atlas combines dense temporal sampling with fine anatomical division of five cortical and subcortical structures. 

View data  |  Documentation  |  Help

Ivy Glioblastoma Atlas

Glioblastoma is an aggressive brain tumor that carries a poor prognosis. The Ivy Glioblastoma Atlas Project (IvyGAP) includes several RNA-Seq and in situ hybridization data sets focused on anatomic structures and cancer stem cell niches. These data sets seek to clarify the relationship between molecular and cellular landscapes of these tumors and their relationships to histologic features routinely used for diagnosis.

Gene expression data  |  Clinical and genomic data  |  SNP data  |  Documentation  |  Help  

Gene expression in the aging brain

The Aging, Dementia and Traumatic Brain Injury (TBI) Study is a detailed neuropathologic, molecular and transcriptomic characterization of brains of control and TBI exposure cases from a unique aged population-based cohort from the Adult Changes in Thought (ACT) study. It includes RNA sequencing data for temporal cortex, parietal cortex, cortical white matter, and hippocampus isolated by macrodissection.

View data  |  Access raw data  |  Documentation  |  Help  

Comprehensive gene expression atlas of the mouse spinal cord

The Allen Spinal Cord Atlas is an interactive database of gene expression mapped across all anatomic segments of the mouse spinal cord at postnatal days 4 and 56. The in situ hybridization data is searchable by gene, age, expression, and in the case of the adult, by the cervical, thoracic, lumbar, sacral, and coccygeal segments, and is accompanied by a high-resolution reference atlas.

View data  |  Documentation  |  Help  

Publications

Key publications below highlight biological findings by researchers at the Allen Institute for Brain Science.  These also include detailed descriptions of methods and analysis protocols. A full list of publications from the Allen Institute is available here.  

Genome-wide atlas of gene expression in the adult mouse brain

For more than a decade, this highly standardized atlas of the adult mouse brain has provided an open, primary data resource for a wide variety of studies involving brain structure and function. Unbiased fine-resolution analysis has identified highly specific gene expression patterns showing remarkable diversity.

Publication  |  Mouse Brain Atlas

Genomic Anatomy of the Hippocampus

Unbiased analysis of hippocampal gene expression data in the Allen Mouse Brain Atlas revealed a large cohort of genes with robust regionalized hippocampal expression. The CA3 compartment can be divided into a set of nine expression domains in the septal/temporal and proximal/distal axes with reciprocal, nonoverlapping boundaries, and evidence of differential connectivity.

Publication  |  Mouse Brain Atlas

Correlated Gene Expression and Target Specificity Demonstrate Excitatory Projection Neuron Diversity

This study describes a systematic approach to identify molecular correlates of specific projection neuron classes in mouse primary somatosensory cortex using existing ISH data mining, marker gene colocalization, and additional retrograde labeling. The combination of gene expression and target specificity imply a great diversity of projection neuron classes matching or surpassing that of GABAergic interneurons.

Publication  |  Mouse Brain Atlas

An anatomically comprehensive atlas of the adult human transcriptome

Brain-wide variation in gene expression strongly reflects the distributions of major cell classes such as neurons, oligodendrocytes, astrocytes, and microglia. The spatial topography of neocortex is strongly reflected in its molecular topography - the closer two cortical regions, the more similar their transcriptomes. 

Publication  |  Human Brain Atlas

Large-scale cellular-resolution gene profiling in human neocortex reveals species-specific molecular signatures

Roughly 80% of genes show consistent expression patterning in mouse and human cortex. Distinct molecular signatures span all major cell types, but primarily suggest a shift from corticosubcortical to more predominant corticocortical communications in the human brain.

Publication  |  Human Brain Atlas  |  Mouse Brain Atlas

Canonical genetic signatures of the adult human brain

Genes with the most reproducible patterns between adult human brains are highly biologically relevant, with enrichment for brain-related annotations such as disease associations. Furthermore, most genes are expressed in one of a relatively few patterns, corresponding to cell types and cellular functions. Research-grade code to reproduce figures is included as supplemental software.

Publication  |  Genetic Geography of the Brain  |  Human Brain Atlas

A High-Resolution Spatiotemporal Atlas of Gene Expression of the Developing Mouse Brain

The timing of peak gene expression patterns corresponds to distinct developmental phenomena associated with a specific brain structures, with midbrain preceding cortical structures. In addition, a transcription factor code of 83 genes uniquely identifies age and brain region.

Publication  |  Developing Mouse Brain Atlas

Transcriptional landscape of the prenatal human brain

This atlas gives a comprehensive view of when and where genes are expressed while the brain is developing, providing insight into dynamic changes in gene expression over time. For example, genes associated with autism are enriched in newly generated excitatory neurons in the cortex.

Publication  |  Microarray data

A comprehensive transcriptional map of primate brain development

Prenatal development is a time of rapid change reflected in gene usage, yet many of the molecular characteristics of the mature brain are achieved surprisingly late in postnatal development. The vast majority -- but not all -- of these patterns are conserved between primate species.

Publication  |  NIH Blueprint NHP Atlas  |  Software

Transcriptional Architecture of the Primate Neocortex

Microarray analysis of individual cortical layers across cortex identified specific molecular signatures for individual cortical layers and areas. Overall, transcriptome-based relationships were related to spatial proximity, being strongest between neighboring cortical areas and between proximal layers. Laminar patterns were more similar between macaque and human compared to mouse.

Publication  |  NIH Blueprint NHP Atlas  |  Access raw data

An anatomic transcriptional atlas of human glioblastoma

Histologically-defined anatomic regions of glioblastoma are shown here to have highly distinct molecular signatures. This, and other yet-to-be-discovered insights in the Ivy Glioblastoma Atlas Project (GAP) could provide new insights into the pathogenesis, diagnosis, and treatment of glioblastoma.

Publication  |  Ivy Glioblastoma Atlas Project  |  IvyGAP Project  |  Access raw data

Neuropathological and transcriptomic characteristics of the aged brain

At the transcriptional level, the aging brain shows remarkable person-to-person variability between dementia and associated pathologies, including examples of resilience to pathology. This study also highlights the importance of controlling for RNA quality when studying the normal and diseased human brain.

Publication  |  Aging, Dementia, and TBI Study  |  Software  |  Access raw data