Mouse Mesoscale Connectivity in Aging and Alzheimer’s Disease

Axonal projections were labeled with stereotaxic injections of viral tracers in mouse models of Alzheimer’s disease and their aged littermates. Whole brain axonal trajectories were quantified using the standardized platform for the Allen Mouse Brain Connectivity Atlas to generate a database of neural projections in aging and diseased mice. Amyloid beta plaques were also labeled in mice with amyloid pathology, and brain-wide plaque density was quantified using an automated pipeline. This dataset can be compared with the projections of young adult (p56) mice in the Allen Mouse Brain Connectivity Atlas to identify differences in anatomy and connectivity that occur in aging and Alzheimer’s disease. 

The data can be downloaded using URLs in this manifest file (330K images listed):

Data Manifest (.XLSX)     Data Manifest ADDENDUM (.csv)    Dataset Summary (.XLSX)     Experiment Details (.XLSX)  

Note: To successfully access all files in the manifest, disregard all rows in "Data Manifest" with a name beginning in "MouseAlz..." and image_series_id="475882459", as these are incorrect.  Instead use the corresponding files in "Data Manifest ADDENDUM" which can be linked to the Experiment Details using the corrected image_series_ids.  All other rows in "Data Manifest" are accurate.

Overview of the data

• Whole brain images and automated detection of axonal projections from stereotaxic injections of fluorescent viral tracers. 

• Whole brain images and automated detection of plaques labeled with methoxy-X04 injections 

• Axonal projections quantified over structures (unionizes) 

• Detected axonal projections and plaques registered to CCFv3 and gridded at 10, 25, and 100 um resolution. 

• Automatically detected dystrophic neurites registered to CCFv3 and gridded at 10, 25, and 100 um resolution. 

• Deformation values from registration 

Related Mouse Lines

These data were generated from stereotaxic injections into the following Alzheimer’s disease and frontotemporal dementia mouse lines. 

Experiments

There are five experiments in this dataset: 

1. Brain-wide mapping 12-15 mo APP/PS1 mice and their littermate controls. 

2. Projection mapping in Cre driver lines crossed to the APP/PS1 mouse line, used to target specific cell classes (12-18 mo). 

3. Targeted mapping of specific regions in various mouse lines and ages. 

4. Selective mapping focused on ENTm in APP/PS1 mice crossed to the rTg4510 tauopathy line. 

5. Target-defined mapping using intersectional viral tracing in APP/PS1 and hAPP-J20 mouse lines. 

Acknowledgment

This project was supported by the National Institute on Aging and the National Institutes of Health by Award Number R01AG047589. Its contents are solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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