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Seattle Alzheimer’s Disease Brain Cell Atlas

Seattle Alzheimer's Disease Brain Cell Atlas (SEA-AD) Study Documentation, Data, and Downloads

The Seattle Alzheimer's Disease Brain Cell Atlas (SEA-AD) consortium includes the Allen Institute for Brain Science, the University of Washington, and Kaiser Permanente Washington Health Research Institute and is supported by the National Institutes on Aging (NIA) grant U19AG060909.

Below you will find information and documentation about the study's data as well as links to raw and processed data.


Documentation, Data, and Downloads

File Size Type Description
Donor metadata 44 KB .xlsx
De-identified Donor metadata (demographic, clinical, cognitive, and neuropathological data)
Link to raw single nucleus data   link
Link to Sage Bionetworks AD Knowledge Portal SEA-AD study page. Controlled access to raw 10x snRNASeq, snATACseq, and Multiome data.
Link to processed single nucleus RNAseq and ATAC-seq data   link
Link to processed single cell data on AWS. Open access to processed 10x snRNAseq and snATAC-seq data.
MRI volumetrics 64KB .xlsx Post mortem MRI brain volumetrics.
Luminex (ABeta and Tau, MTG) 24KB .xlsx Luminex data: ABeta and Tau measurements for Middle Temporal Gyrus (MTG).
Quantitative neuropathology summary data (MTG) 253KB .csv Measurements of Abeta, pTau, pTDP43, a-synuclein, Neun+ cells, IBA1+ cells, and GFAP+ cells from quantitative analysis of stained neuropathology images from Middle Temporal Gyrus (MTG).
Neuropathology: raw and analyzed/annotated image files   link Link to neuropathology image files on AWS. Download raw and quantified (stained images annotated and masked via the HALO image analysis software from Indica Labs).


File Size Type Description
SEA-AD cohort information 99KB .pdf
SEA-AD cohort selection information, inclusion/exclusion criteria
SEA-AD cohort metadata summary graphs 514KB .pdf
Graphs summarizing the SEA-AD cohort's age at death, sex, APOE4 status, cognitive status, CERAD score, ADNC score, Braak stage, and Thal phase.
Transcriptomics and epigenomics data generation 95KB .pdf Methods for 10x snRNASeq, snATACseq, and Multiome data generation and RNA Integrity Number (RIN) determination
Transcriptomics: Post-sequencing data analysis 153KB .pdf Methods for transcriptomics data analysis. Information on development of web tools, which present transcriptomic data. 
Donor metadata brief descriptions 90KB .pdf Brief descriptions for the donor metadata elements.
Donor metadata citations 123KB .pdf
Citations for select donor metadata elements 
Consensus clinical diagnosis 136KB .pdf Information on clinical AD detection and consensus clinical diagnosis generation.
Dissection, MRI, neuropathological analysis 108KB .pdf Citation for dissection, ex vivo MRI, tissue preparation, and neuropathological analysis.
Information on processed single cell profiling data 217KB .pdf Methods information and tutorial for processed single cell profiling data available on AWS
Information on neuropathology images and image analysis data 764KB .pdf Methods information and tutorial for neuropathology images and image analysis data available on AWS