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Reference Genome Files (.gtf)

 

This page includes General Transfer Format (.gtf) or related files which allow one to reproduce the alignment from raw (fastq) sequencing data to a specific version of the genome/transcriptome in RNA-seq analysis. Most individuals wanting to use processed versions of Allen Institute sequencing data (e.g., cell x gene matrices) can safely ignore this page. The top table shows a data type-centric view of these files, while the bottom table shows at project-centric view of the same files. In both tables links are colored in blue for convenience.

 

In the tables below, 'SSv4' indicates any single cell or single nucleus RNA-sequencing or Patch-Seq data that was processed using the SMARTerV4 method, while '10x' indicates any single cell or single nucleus RNA-sequencing data processed using 10x Genomics single-omics or multiomics methods. All mouse and human single cell or single nucleus RNA-sequencing data was aligned to some version of the GRCm38 and GRCh38 genomics, respectively, although file formats and transcriptomics versions differ between rows. NA in the table means no data has every been generated in the relevant slot, while "in process" means that data has been generated but currently is not included in any Allen Brain Map tools. Finally, data from several projects with data generated prior to 2022 (including Patch-seq data) were originally processed using one transcriptome version and have since been converted into the current version. In this case, refer to the relevant Allen Brain Map or scientific public to determine which genome/transcriptome version was used. Abbreviations: GBM = Ivy Glioblastoma Atlas Project; TBI = Aging, Dementia and Traumatic Brain Injury Study; WHB = Whole human brain atlas included in ABC Atlas (May 2024); CTKE = Cell Type Knowledge Explorer; LGN = Lateral Geniculate Nucleus (part of Comparative LGN project)

 

Reference files by category and species

 

Data sets used Human Mouse Marmoset Macaque Other mammals
10x processed using CellRanger V6 (~2022 - current) GRCh38/gencode.v32 mm10/genecode.vM23 in process in process in process
SSv4 (~2022 - current; conversion date same as 10x) GRCh38/gencode.v32 mm10/genecode.vM23 in process in process in process
10x processed using CellRanger V3 (start - ~2021) GRCh38.p2/pre-mRNA mm10/10x pre-mRNA v3.0.0 NA NA NA
SSv4 (start - ~2021; conversion date same as 10x) GRCh38.p2 GRCm38.p3 NA ref_Mmul_10_top_level (LGN) NA
Projects with data processed by collaborators GRCh38.p13/gencode.v35 (WHB) | Gencode v10 (BrainSpan) NA mCalJa1.2.pat.X (CTKE) NA NA
Historical bulk RNA-Seq data sets GRCh38.p2 (TBI) | GRCh37.p5 (GBM) NA NA NA NA

 

Reference files by data set

 

Project name Species Year Modality Link to data set or product Link to manuscript(s) Reference file
Seattle Alzheimer’s Disease Brain Cell Atlas Human Ongoing 10x https://sea-ad.org https://doi.org/10.1101/2023.05.08.539485 GRCh38/gencode.v32
Human Patch-seq Human Ongoing SSv4 https://portal.brain-map.org/explore/classes/multimodal-characterization (Manuscripts are listed in link to product) GRCh38/gencode.v32 (data in some manuscripts use GRCh38.p2)
Mouse Patch-seq Mouse Ongoing SSv4 https://portal.brain-map.org/explore/classes/multimodal-characterization (Manuscripts are listed in link to product) mm10/genecode.vM23 (data in some manuscripts use GRCm38.p3)
Human Whole Brain Atlas Human 2023 10x https://portal.brain-map.org/atlases-and-data/bkp/abc-atlas https://doi.org/10.1126/science.add7046 GRCh38.p13/gencode.v35
Mouse Whole Brain Atlas Mouse 2023 10x https://portal.brain-map.org/atlases-and-data/bkp/abc-atlas https://doi.org/10.1038/s41586-023-06812-z mm10/genecode.vM23
MTG - 10x SEA-AD (2022) Human 2022 10x https://portal.brain-map.org/atlases-and-data/rnaseq/human-mtg-10x_sea-ad https://doi.org/10.1101/2023.05.08.539485 GRCh38/gencode.v32
Marmoset - M1 (CTKE) Marmoset 2021 10x https://portal.brain-map.org/atlases-and-data/bkp/cell-type-knowledge-explorer https://doi.org/10.1038/s41586-021-03465-8 mCalJa1.2.pat.X
Mouse - M1 (CTKE) Mouse 2021 10x https://portal.brain-map.org/atlases-and-data/bkp/cell-type-knowledge-explorer https://doi.org/10.1038/s41586-021-03500-8 mm10/10x pre-mRNA v3.0.0
M1 - 10x genomics (2020) (Transcriptomics Explorer and CTKE) Human 2020 10x https://portal.brain-map.org/atlases-and-data/rnaseq/human-m1-10x https://doi.org/10.1038/s41586-021-03465-8 GRCh38.p2/pre-mRNA
Whole Cortex & Hippocampus - 10x genomics (2020) Mouse 2020 10x https://portal.brain-map.org/atlases-and-data/rnaseq/mouse-whole-cortex-and-hippocampus-10x https://doi.org/10.1016/j.cell.2021.04.021 mm10/10x pre-mRNA v3.0.0
Whole Cortex & Hippocampus - SMART-seq (2019) Mouse 2019 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/mouse-whole-cortex-and-hippocampus-10x https://doi.org/10.1016/j.cell.2021.04.021 GRCm38.p3
Multiple Cortical Areas - SMART-seq (2019) Human 2019 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/human-multiple-cortical-areas-smart-seq https://doi.org/10.1126/science.adf6812 GRCh38.p2
MTG - SMART-seq (2018) Human 2018 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/human-mtg-smart-seq https://doi.org/10.1038/s41586-019-1506-7 GRCh38.p2
V1 & ACC - SMART-seq (2018) Human 2018 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/human-v1-acc-smart-seq https://doi.org/10.1126/science.adf6812 GRCh38.p2
VISp & ALM - SMART-seq (2018) Mouse 2018 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/mouse-v1-and-alm-smart-seq https://doi.org/10.1038/s41586-018-0654-5 GRCm38.p3
ACA & MOp - SMART-seq (2018) Mouse 2018 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/mouse-aca-and-mop-smart-seq https://doi.org/10.1016/j.cell.2021.04.021 GRCm38.p3
Human - LGN (2018) Human 2018 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/comparative-lgn https://doi.org/10.7554/eLife.64875 GRCh38.p2
Macaque - LGN (2018) Macaque 2018 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/comparative-lgn https://doi.org/10.7554/eLife.64875 ref_Mmul_10_top_level
Mouse - LGN (2018) Mouse 2018 SSv4 https://portal.brain-map.org/atlases-and-data/rnaseq/comparative-lgn https://doi.org/10.7554/eLife.64875 GRCm38.p3
Aging, Dementia, and TBI Study Human 2017 Other https://aging.brain-map.org/ https://doi.org/10.7554/eLife.31126 GRCh38.p2
Ivy Glioblastoma Atlas Project Human 2015 Other https://glioblastoma.alleninstitute.org https://doi.org/10.1126/science.aaf2666 GRCh37.p5
BrainSpan Atlas of the Developing Human Brain Human 2014 Other https://www.brainspan.org https://doi.org/10.1126/science.aat7615 Gencode v10